An Introduction to Behavioural Ecology by Nicholas B. Davies & John R. Krebs & Stuart A. West

Author:Nicholas B. Davies & John R. Krebs & Stuart A. West [Davies, Nicholas B.]
Language: eng
Format: mobi
Publisher: Wiley
Published: 2012-02-17T05:00:00+00:00

BOX 9.1 USING DNA PROFILES TO ASSIGN PARENTAGE

In 1985, Alec Jeffreys and colleagues discovered that there was enormous genetic variability which could be used to assign paternity and maternity with great precision. Since then, methods for DNA profiling have been refined and they have revolutionized field studies by linking behaviour to individual reproductive success. An individual’s DNA is isolated from a tissue sample (e.g. blood) or non-invasively, for example by analysis of epithelial cells on dung (these cells are sloughed off as faeces pass through the intestine). Microsatellites, or Simple Sequence Repeats, are stretches of DNA that contain short nucleotide repeats of two to six base pairs. The microsatellite below contains a two base pair (dinucleotide) repeat of the bases G and T.

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